High-throughput optofluidic system for the laser microsurgery of oocytes.

نویسندگان

  • Charlie Chandsawangbhuwana
  • Linda Z Shi
  • Qingyuan Zhu
  • Mark C Alliegro
  • Michael W Berns
چکیده

This study combines microfluidics with optical microablation in a microscopy system that allows for high-throughput manipulation of oocytes, automated media exchange, and long-term oocyte observation. The microfluidic component of the system transports oocytes from an inlet port into multiple flow channels. Within each channel, oocytes are confined against a microfluidic barrier using a steady fluid flow provided by an external computer-controlled syringe pump. This allows for easy media replacement without disturbing the oocyte location. The microfluidic and optical-laser microbeam ablation capabilities of the system were validated using surf clam (Spisula solidissima) oocytes that were immobilized in order to permit ablation of the 5 μm diameter nucleolinus within the oocyte nucleolus. Oocytes were the followed and assayed for polar body ejection.

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عنوان ژورنال:
  • Journal of biomedical optics

دوره 17 1  شماره 

صفحات  -

تاریخ انتشار 2012